Matt Damon as Jason Bourne
Joan Allen as Pamela "Pam" Landy: a CIA Deputy Director and Task Force Chief, pursues Bourne after her operation goes badly
Brian Cox as Ward Abbott: a CIA Deputy Director formerly in charge of Treadstone, is actually in league with Gretkov for stolen CIA money
Julia Stiles as Nicky Parsons: formerly Bourne's Treadstone contact, is taken from her post-Treadstone assignment to assist in the search for Bourne
Karl Urban as Kirill: a Russian secret service agent and an expert assassin who is working for Gretkov as a favor to Abbott
Karel Roden as Gretkov: Kirill's employer, collaborating with Abbott for stolen CIA money
Franka Potente as Marie Helena Kreutz: Bourne's girlfriend.
Gabriel Mann as Danny Zorn: formerly assigned to Treadstone headquarters, is now on Abbott's staff